Microbial Production of Chiral Hydroxy Esters and Their Analogs: Biocatalytic Reduction of Carbonyl Compounds by Actinobacteria, Agromyces and Gordonia Strains
Autor: | Ayaka Shuto, Noriyoshi Masuoka, Kohji Ishihara, Daisuke Uesugi, Chiharu Kuboki, Nobuyoshi Nakajima, Takumu Mishima, Manami Inoue, Natsumi Adachi, Kazuya Okamoto, Hiroki Hamada |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
food.ingredient 030102 biochemistry & molecular biology biology Carbonyl Reductase Chemistry Stereochemistry Gordonia biology.organism_classification Microbacteriaceae Actinobacteria Agromyces 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology food Biocatalysis Amide General Materials Science Stereoselectivity |
Zdroj: | Advances in Enzyme Research. :15-25 |
ISSN: | 2328-4854 2328-4846 |
Popis: | We screened 15 Agromyces strains from the Microbacteriaceae family and 16 Gordonia strains from the Gordoniaceae family to investigate their biocatalytic ability to reduce carbonyl compounds. Two Agromyces strains (A. soli NBRC109063 and A. humatus NBRC109085) and two Gordonia strains (G. hydrophobica NBRC16057 and G. malaquae NBRC108250) grew well in 230 medium. The stereoselective reduction of various carbonyl compounds using these four strains was investigated. We discovered that these strains can reduce aliphatic and aromatic α-keto esters and an aromatic α-keto amide. On the basis of the conversion rate and stereoselectivity of the alcohols produced, G. hydrophobica NBRC16057 is a potential biocatalyst for the stereoselective reduction of α-keto esters and an aromatic α-keto amide to the corresponding chiral alcohols. Our results also suggest that the reduction of ethyl 2-methylacetoacetate by wet G. hydrophobica NBRC16057 cells in the presence of L-glutamate is useful for the production of chiral ethyl 3-hydroxy-2-methylbutanoate. |
Databáze: | OpenAIRE |
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