Enhanced Fusarium oxysporum f. sp. tuberosi Resistance in Transgenic Potato Expressing a Rice GLP Superoxide Dismutase Gene
Autor: | Nadia Majeed, Bushra Javaid, Farah Deeba, David S. Douches, S. M. Saqlan Naqvi |
---|---|
Rok vydání: | 2018 |
Předmět: |
0106 biological sciences
0301 basic medicine Oryza sativa Transgene fungi Oxalate oxidase activity Wild type food and beverages Plant Science Biology Plant disease resistance biology.organism_classification 01 natural sciences Microbiology Superoxide dismutase 03 medical and health sciences 030104 developmental biology Fusarium oxysporum biology.protein Gene family Agronomy and Crop Science 010606 plant biology & botany |
Zdroj: | American Journal of Potato Research. 95:383-394 |
ISSN: | 1874-9380 1099-209X |
DOI: | 10.1007/s12230-018-9639-z |
Popis: | Germin like proteins (GLPs) are a large group of related and ubiquitous plant proteins which are considered to be involved in different processes important for plant development and defense. Multiple functional copies of this gene family have been reported in a number of species (wheat, barley, rice, soybean mosses and liverwort), and their role is being evaluated by gene regulation studies and transgenic approaches. To analyze the role of a rice (Oryza sativa) root expressed germin like protein1 OsRGLP1, for its antifungal activity, transgenic potato plants were developed. These transgenic potato plants were molecularly characterized and biologically assessed after inoculation with Fusarium oxysporum f. sp. tuberosi. Functional analysis showed high accumulation of H2O2, increased Superoxide Dismutase (SOD) activity and no oxalate oxidase activity (OxO) in transgenics in comparison to nontransformed control. This increased SOD activity, resistance to heat and sensitivity to H2O2 suggest it is a Fe-like SOD. OsRGLP1 expression in potato plants exhibited enhanced resistance in comparison to nontransformed wild type plants suggesting its role in providing protection against Fusarium oxysporum f. sp. tuberosi through elevated SOD level. Overall, results suggest that OsRGLP1 is a candidate for the engineering of potato plants with increased fungal tolerance however, the greater height and tuber number was observed. This phenotype associated with the resistance needs to be evaluated to determine if this is a positive or negative feature. |
Databáze: | OpenAIRE |
Externí odkaz: | |
Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |