Genetically Engineered Fd Viruses for Site Specific Material Binding
| Autor: | Taner Arslan, Nuriye Korkmaz |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
biology Genetically engineered Atomic force microscopy Chemistry Biomedical Engineering Bioengineering 02 engineering and technology Quartz crystal microbalance Coat protein 021001 nanoscience & nanotechnology biology.organism_classification law.invention 03 medical and health sciences 030104 developmental biology law Recombinant DNA Biophysics 0210 nano-technology Bacteria |
| Zdroj: | BioNanoScience. 9:862-872 |
| ISSN: | 2191-1649 2191-1630 |
| Popis: | Filamentous bacteriophages are viruses infecting only bacteria. In this study, filamentous fd viruses were genetically engineered to display specific gold- (V5) and platinum-binding (p7A) peptides on the head part (P9 minor coat protein). Gold-binding properties of recombinant p9V5, p9P7A, and wild-type fd viruses were investigated by Quartz Crystal Microbalance (QCM), scanning electron microscopy (SEM), atomic force microscopy (AFM), energy dispersive X-ray spectroscopy (EDX), and UV-Vis spectroscopy analyses. Displaying only 5 copies of V5 peptides on head part of viruses resulted in site-specific Au-binding. Surprisingly, p9p7A viruses, as well, showed enhanced gold-binding properties with respect to wild-type fd viruses. Filamentous fd viruses are good candidates of genetically programmable biotemplates for mineralization and metallization studies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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