Proteoglycans in Predentin: The Last 15 Micrometers Before Mineralization
| Autor: | Laurent Ameye, K. Palmier, O. Rapoport, G. Embery, Michel Goldberg, Rachel C. Hall, Dominique Septier, Marian F. Young |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Decorin
Keratan sulfate Biglycan Cell Biology Biochemistry Dermatan sulfate Cell biology carbohydrates (lipids) chemistry.chemical_compound stomatognathic system Rheumatology chemistry Immunochemistry Dentinogenesis Orthopedics and Sports Medicine Chondroitin sulfate Molecular Biology Extracellular matrix organization |
| Zdroj: | Connective Tissue Research. 44:184-188 |
| ISSN: | 1607-8438 0300-8207 |
| DOI: | 10.1080/03008200390152304 |
| Popis: | Small leucine-rich proteoglycans (SLRPs) regulate extracellular matrix organization. In order to investigate the distribution and potential functions of decorin, biglycan (BGN), and fibromodulin (3 SLRPs, potentially related to dentinogenesis), we performed light and electron immunochemistry on teeth from rats, and on wild-type and biglycan knockout mice (BGN KO). Immunohistochemical data demonstrate that chondroitin sulfate/dermatan sulfate (CS/DS) and keratan sulfate (KS) distributions displayed reverse gradients in predentin. The decrease of CS/DS labeling from the proximal to the distal predentin contrasted with the sharp decorin increase observed in the distal predentin near the predentin/dentin transition, an effect possibly attributable to the deglycosylation action of stromelysin-1. In contrast, BGN concentration was apparently constant throughout the whole predentin. Additional immunolabelings showed, for the first time, the presence of fibromodulin in predentin. Compared with the wild-type mouse... |
| Databáze: | OpenAIRE |
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