Crystallization and some characterization of flammulin purified from the fruit bodies of Flammulina velutipes
| Autor: | ChangKai Zhang, YaLi Chen, Feng Gong, Ming Gong |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Gel electrophoresis
Environmental Engineering Chromatography biology Renewable Energy Sustainability and the Environment Isoelectric focusing Bioengineering General Medicine Polyethylene glycol Fractionation biology.organism_classification law.invention chemistry.chemical_compound Isoelectric point chemistry law Crystallization Waste Management and Disposal Ammonium sulfate precipitation Flammulina |
| Zdroj: | Bioresource Technology. 64:153-156 |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/s0960-8524(97)00075-8 |
| Popis: | From the aqueous extract of fruit bodies of Flammulina velutipes, Flammulin, an antitumor substance, was purified to homogeneity judged by SDS-polyacrylamide gel electrophoresis. The purification procedure included ammonium sulfate precipitation, ethanol fractionation, DEAE-Sephadex A50 anion-exchange chromatography, crystallization and recrystallization. Flammulin was crystallized by microdialysis against 12% polyethylene glycol 6000, in 20 m m sodium phosphate buffer (pH 7.0). The crystals were rhombohedron-shaped and easy to melt when the crystallization conditions were changed. With a molecular mass of 24000, Flammulin is characterized by its lack of methionine. Amino acid composition analysis showed that the acidic amino acids were in greater number than the basic amino acids, but the isoelectric point detected by analytical isoelectric focusing was at pH 8.9. |
| Databáze: | OpenAIRE |
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