Murine leukemia inhibitory factor enhances retroviral-vector infection efficiency of hematopoietic progenitors
| Autor: | FA Fletcher, DE Williams, C Maliszewski, D Anderson, M Rives, JW Belmont |
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| Rok vydání: | 1990 |
| Předmět: | |
| Zdroj: | Blood. 76:1098-1103 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v76.6.1098.bloodjournal7661098 |
| Popis: | We have investigated the in vitro effects of the cytokine leukemia inhibitory factor (LIF) on normal murine hematopoietic progenitors by measuring recovery and retroviral vector infection efficiency of 13-day posttransplant, spleen-colony-forming cell (CFU-S 13) in short-term culture. Up to a twofold increase in CFU-S13 recovery was observed, from 9.7 x 10(-5) cells in untreated controls to 17.8 to 19.5 x 10(-5) cells, depending on the concentration of LIF. Histologic analysis of spleen colonies from control and LIF-treated marrows demonstrated that there was no detectable alteration in the differentiative potential of CFU-S13. The efficiency of CFU-S13 infection was increased from 15% in untreated controls to 84% to 91% in LIF-treated marrows. Analysis of proviral integration sites in spleen colonies indicated that some CFU- S13 precursors were infected in the LIF-treated marrows. |
| Databáze: | OpenAIRE |
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