Flow injection amperometric and chemiluminescence individual and simultaneous determination of lysine and glucose with immobilized lysine oxidase and glucose oxidase
| Autor: | Alan Townshend, Ala'ddin M. Almuaibed |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Detection limit
chemistry.chemical_classification Oxidase test Chromatography biology Chemistry Metal ions in aqueous solution Lysine complex mixtures Biochemistry Amperometry Analytical Chemistry law.invention Amino acid law biology.protein Environmental Chemistry Glucose oxidase Spectroscopy Chemiluminescence |
| Zdroj: | Analytica Chimica Acta. 338:149-154 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/s0003-2670(96)00439-4 |
| Popis: | Flow injection amperometric and chemiluminescence methods for the individual and simultaneous determination of lysine and glucose by using lysine oxidase and glucose oxidase are described in this paper. The calibration graphs for lysine are linear over the range 1 × 10 −5 − 1.6 × 10 −4 and 2.05 × 10 −7 − 2.75 × 10 −5 M for amperometric and chemiluminescence detection respectively. The detection limits (3σ) for lysine are 5 × 10 −6 M with amperometric and 4.0 × 10 −8 M with chemiluminescence detection. The sample throughput rates are 80 and 100 h −1 , respectively. Lysine and glucose concentrations can be monitored simultaneously in the range 1.5 − 9 × 10 −5 M and 7 × 10 −7 −8 × 10 −6 M with amperometric and chemiluminescence detection respectively. The interference effects of some amino acids and metal ions are examined. An on-line cation-exchange minicolumn is inserted into the chemiluminescence manifold to remove the interference effect of Fe 2+ and Cu 2+ . |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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