Multifunctional Polyelectrolyte Microparticles for Oral Insulin Delivery
| Autor: | N. G. Balabushevich, E. V. Mikhalchik, E. D. Shibanova, Dmitry Volodkin, M. A. Pechenkin |
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| Rok vydání: | 2013 |
| Předmět: |
Protease
Polymers and Plastics medicine.diagnostic_test medicine.medical_treatment Proteolysis Insulin Bioengineering Pharmacology Polyelectrolyte Bioavailability Biomaterials Chitosan chemistry.chemical_compound chemistry Oral administration Materials Chemistry medicine Protease inhibitor (pharmacology) Biotechnology |
| Zdroj: | Macromolecular Bioscience. 13:1379-1388 |
| ISSN: | 1616-5187 |
| Popis: | Multicomponent insulin-containing microparticles are prepared by layer-by-layer assembly of dextran sulfate and chitosan on the core of protein-polyanion complex with or without protease inhibitors. Oral bioavailability of the encapsulated insulin is improved due to the cumulative effect of each component. A physico-chemical study shows that the particle design allows adjustment of the pH-dependent profile of the insulin release, as well as mucoadhesive properties and Ca(2+) binding ability of the microparticles. Supplementing the microparticles with 2-3% protease inhibitors fully prevents proteolysis of human insulin. The pharmacological effect of microencapsulated insulin in doses 50-100 IU kg(-1) is demonstrated in chronic experiments after oral administration to diabetic rats fed ad libitum. |
| Databáze: | OpenAIRE |
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