Methotrexate-conjugated AgInS2/ZnS quantum dots for optical imaging and drug delivery
| Autor: | Keng Liang Ou, Shin Hwa Tzing, Wei Xiang Lin, Jem-Kun Chen, Hsiao Ping Fang, Jia-Yaw Chang, Pei Jung Wu |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Materials science
biology Mechanical Engineering technology industry and agriculture Nanotechnology Conjugated system equipment and supplies Condensed Matter Physics biology.organism_classification Combinatorial chemistry Nanomaterials HeLa chemistry.chemical_compound chemistry Mechanics of Materials Drug delivery General Materials Science MTT assay Viability assay Cytotoxicity Carbodiimide |
| Zdroj: | Materials Letters. 128:412-416 |
| ISSN: | 0167-577X |
| Popis: | The ability to conjugate drugs with quantum dots (QDs) offers excellent prospects in pharmacotherapy for the improvement of diagnosis and treatment of human diseases. Here, we report a facile strategy to prepare dual-functional nanomaterials consisting of QDs and an anticancer drug. Hydrophilic AgInS2/ZnS QDs were obtained through an ultrasonication process by introducing the amphiphilic polymer, poly(maleic anhydride-alt-1-octadecene) (PMAO). Subsequently, the anticancer drug methotrexate (MTX) was covalently coupled to the surface of the AgInS2/ZnS QDs by carbodiimide chemistry. The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] cell proliferation assay indicated that these hydrophilic PMAO-coated AgInS2/ZnS QDs exhibited negligible cytotoxicity. Human cervical (HeLa) cancer cells had >80% cell viability after 24 h at concentrations of up to 300 μg/mL. Confocal laser scanning microscopy revealed that the AgInS2/ZnS QDs conjugated with MTX were taken up effectively by HeLa cells. In addition, this nanomaterial was shown to have a dose-dependent antiproliferative effect in cancer cells by the MTT assay. These important features suggest that AgInS2/ZnS QDs conjugated with MTX might be a novel candidate for cancer therapeutics and diagnosis in future. |
| Databáze: | OpenAIRE |
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