The bacA gene, which determines bacitracin susceptibility in Streptococcus pneumoniae and Staphylococcus aureus, is also required for virulence The GenBank accession number for the sequence reported in this paper is AF228662

Autor:	David J. Holmes, Nicola G. Wallis, Alexander P. Bryant, Karen A. Ingraham, John Broskey, Stewart C. Pearson, Alison F. Chalker, Joanna Bryant, R. Dwayne Lunsford
Rok vydání:	2000
Předmět:	Mutant Virulence Mutagenesis (molecular biology technique) Bacitracin Undecaprenol kinase Biology medicine.disease_cause Microbiology Staphylococcus aureus Streptococcus pneumoniae medicine Escherichia coli medicine.drug
Zdroj:	Microbiology. 146:1547-1553
ISSN:	1465-2080 1350-0872
DOI:	10.1099/00221287-146-7-1547
Popis:	Homologues of Escherichia coli bacA, encoding extremely hydrophobic proteins, were identified in the genomes of Staphylococcus aureus and Streptococcus pneumoniae. Allelic replacement mutagenesis demonstrated that the gene is not essential for in vitro growth in either organism, and the mutants showed no significant changes in growth rate or morphology. The Staph. aureus bacA mutant showed slightly reduced virulence in a mouse model of infection and an eightfold increase in bacitracin susceptibility. However, a Strep. pneumoniae bacA mutant was highly attenuated in a mouse model of infection, and demonstrated an increase in susceptibility to bacitracin of up to 160000-fold. These observations are consistent with the previously proposed role of BacA protein as undecaprenol kinase.
Databáze:	OpenAIRE
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