Aspects of the capillary GC analysis of all-trans- and 13-cis-acitretin

Autor:	Evelyne Meyer, Pat Sandra, Andreas De Leenheer
Rok vydání:	1992
Předmět:	Chromatography General Chemical Engineering Metabolite Extraction (chemistry) Mass spectrometry High-performance liquid chromatography Acitretin chemistry.chemical_compound chemistry medicine Gas chromatography Selectivity Derivatization medicine.drug
Zdroj:	Journal of High Resolution Chromatography. 15:637-640
ISSN:	1521-4168 0935-6304
DOI:	10.1002/jhrc.1240151003
Popis:	The capillary gas chromatographic (CGC) analysis of the der-matological drug trans-acitretin (NeotigasonR) and its cis metabolite is described. Separation of the methyl ester derivatives can be achieved on a 90% biscyanopropylsiloxane phase. The importance of using cold on-column injection and short, thin film capillary columns is discussed. For patients treated with the prodrug of acitretin, etretinate (TigasonR), i.e. the ethyl ester of Neotigason, three compounds have to be separated. Selectivity tuning is required for successful CGC separation. An alternative can be found in the selectivity of ion monitoring mass spectroscopy. Analysis of plasma samples involves liquid-liquid extraction, a derivatization step, and HPLC purification.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9dd19663da9a708180975460e45a6409 https://doi.org/10.1002/jhrc.1240151003 Zobrazit plný text záznamu Plný text