Folate Deficiency Induces Neural Stem Cell Apoptosis by Increasing Homocysteine In Vitro
| Autor: | Guowei Huang, John X. Wilson, Zhi-Hong Tian, Da-Lin Ren, Xumei Zhang |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Nutrition and Dietetics
Homocysteine medicine.diagnostic_test Cell growth Clinical Biochemistry Cell Medicine (miscellaneous) Nestin Biology Neural stem cell chemistry.chemical_compound medicine.anatomical_structure nervous system Biochemistry chemistry Western blot Apoptosis Cancer research medicine biological phenomena cell phenomena and immunity Stem cell reproductive and urinary physiology |
| Zdroj: | Journal of Clinical Biochemistry and Nutrition. 45:14-19 |
| ISSN: | 1880-5086 0912-0009 |
| Popis: | Cellular events for neural progenitor cells, such as proliferation and differentiation, are regulated by multiple intrinsic and extrinsic cell signals. Folate plays a central role in central nervous system development, so folate, as an extrinsic signal, may affect neural stem cell (NSC) proliferation and differentiation. In the present study, we investigated the effects of folate deficiency on the cell proliferation, cell apoptosis and homocysteine concentrations in NSCs. NSCs were isolated from fetal rats and identified as NSCs by their expression of immunoreactive nestin. Cell proliferation was quantitated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Apoptotic cells were detected and confirmed by flow cytometric analysis. We measured homocysteine concentrations in NSCs by high performance liquid chromatography and detected the expression of caspase-3 by western blot method. Folate deficiency not only decreased cell proliferation, but also increased the apoptotic rate of NSCs as demonstrated by the increased expression of early apoptotic markers such as caspase-3, compared to control group (p |
| Databáze: | OpenAIRE |
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