Effect ofLegionella pneumophilasonicate on killing ofListeria monocytogenesby human polymorphonuclear neutrophils and monocytes
| Autor: | J. M. Bangsborg, Catherine Rechnitzer, Geoffrey H. Shand |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Microbiology (medical)
Lipopolysaccharide biology Legionella Intracellular parasite Monocyte Phagocytosis General Medicine medicine.disease_cause biology.organism_classification Legionella pneumophila Pathology and Forensic Medicine Microbiology chemistry.chemical_compound medicine.anatomical_structure chemistry Listeria monocytogenes medicine Listeria Immunology and Allergy |
| Zdroj: | APMIS. 101:249-256 |
| ISSN: | 1600-0463 0903-4641 |
| Popis: | Legionella pneumophila shares with other intracellular pathogens the ability to resist intracellular killing within phagocytes. An increasing number of cellular components of L. pneumophila are proposed as pathogenic factors of the organism. At the site of infection, the phagocytic cells will be exposed to bacterial components, either expressed on the surface of the organisms or released in the environment upon cell lysis. In this study, we have investigated the effect of water-soluble bacterial components present in L. pneumophila sonicate on the phagocytosis and bactericidal activity of human polymorphonuclear neutrophils and monocytes. Preincubation of neutrophils with L. pneumophila sonicate did not affect phagocytosis of L. monocytogenes, whereas Listeria killing was significantly inhibited at sonicate concentrations of 1 and 2 mg/ml. The phenol phase of a phenol-water extraction, containing most of the lipopolysaccharide (LPS), had no inhibitory effect on the listericidal activity of neutrophils. Killing of Listeria by monocytes was inhibited in a similar manner. The inhibitory activity was mainly recovered in the sonicate fraction above 100 kDa, suggesting that components organized in larger molecular complexes are most likely to represent the inhibitory factors. The inhibitory activity of L. pneumophila sonic extract appears to be related to inhibition of killing mechanisms since uptake of Listeria was not affected by the sonicate. Our observations indicate that as Legionella infection progresses, bacterial components liberated by cell lysis could exert a detrimental effect on the antimicrobial function of phagocytes, stressing the importance of early treatment of Legionnaires' disease to reduce bacterial numbers in the infected tissues. |
| Databáze: | OpenAIRE |
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