| Autor: |
Hugo Gonzalez, Henry Hebel, Sriram Ramakrishnan, Heather Attra |
| Rok vydání: |
2004 |
| Předmět: |
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| Zdroj: |
Molecular Therapy. 9:S310-S311 |
| ISSN: |
1525-0016 |
| Popis: |
Cost-effective production of commercial-scale batches of pharmaceutical-grade plasmid DNA remains a challenge for the maturing industry of plasmid-based biologics. A process is required that meets the quality standards for human pharmaceutical products and yet is economically suitable for use in veterinary medicine or as an in-process product for viral therapeutic production. Previously described scaleable cell lysis requires a substantial capital investment. Also, these techniques contaminate the process stream with unnecessary impurity levels. A simple, effective purification strategy requires elimination of all animal-derived enzymes, without adding costly column-based chromatography steps. The method described herein has produced multi-gram batches of pharmaceutical-grade plasmid at a fraction of the cost of previously described methods. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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