Nitric oxide metabolites in induced sputum: a marker of airway inflammation in asthmatic subjects
| Autor: | Inseon S. Choi, C. S. Park, An-Soo Jang, Joo-Shil Lee, Kong-Soo Lee, Seo Jp, Hae-Sim Park, Sun-Ok Lee, Park Ko, Yang Sw |
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| Rok vydání: | 1999 |
| Předmět: |
medicine.medical_specialty
Saliva Eosinophil cationic protein business.industry Metabolite Immunology Eosinophil medicine.disease Gastroenterology chemistry.chemical_compound FEV1/FVC ratio medicine.anatomical_structure chemistry Internal medicine medicine Immunology and Allergy Sputum medicine.symptom business Interleukin 5 Asthma |
| Zdroj: | Clinical & Experimental Allergy. 29:1136-1142 |
| ISSN: | 0954-7894 |
| Popis: | Background and objective The role of nitric oxide (NO) needs to be further clarified in allergic inflammation. This study was designed to investigate the relationships between NO metabolites and eosinophil count, eosinophil cationic protein (ECP), interleukin (IL)-5 in induced sputum from asthmatics. Methods Hypertonic saline-induced sputum was obtained in 25 asthmatic subjects, among which 13 patients were examined before and after anti-asthmatic medications including steroid preparations. Ten normal subjects were enrolled as controls. Fresh expectorated sputum separated from saliva was treated with equal volume of dithiothreitol 0.1%, cytospined for cell count, and the supernatant was collected for biochemical assay. NO metabolites were assayed by using modified Griess reaction. ECP was measured by fluoroimmunoassay, and detected IL-5 by a sandwich ELISA. Results Asthmatic subjects, compared with controls, had significantly higher concentration of NO metabolites (1035.4 ± 125.3 vs 557.2 ± 101.5 μmol/L, P |
| Databáze: | OpenAIRE |
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