Improvement of versatile peroxidase activity and stability by a cholinium-based ionic liquid
| Autor: | Behdad Delavari, Hossein Lanjanian, Ali Akbar Saboury, Fatemeh Mamashli, Marjan Sabbaghian, Morteza Hosseini, Jalil Badraghi |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Circular dichroism
02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics Photochemistry 01 natural sciences Fluorescence Atomic and Molecular Physics and Optics 0104 chemical sciences Electronic Optical and Magnetic Materials chemistry.chemical_compound Molecular dynamics chemistry Ionic liquid Materials Chemistry Molecule Physical and Theoretical Chemistry 0210 nano-technology Versatile peroxidase Heme Spectroscopy Choline chloride |
| Zdroj: | Journal of Molecular Liquids. 272:597-608 |
| ISSN: | 0167-7322 |
| DOI: | 10.1016/j.molliq.2018.09.128 |
| Popis: | Deep eutectic solvents (DESs) have been introduced as green solvents and less expensive alternatives for ionic liquids. In the current study, effects of a cholinium based DES, Choline chloride:Glycerol (ChCl:G), on function and structure of versatile peroxidase (VP) were studied. Functional analysis of VP in the presence of various concentrations of ChCl:G revealed lower Km values at lower concentrations and improved activity in neutral pH. According to our results, VP presented higher exposure of hydrophobic patches and formed protein assemblies as revealed by extrinsic fluorescence, fluorescence lifetime, and anisotropy measurements. Circular dichroism and UV–vis spectroscopies demonstrated secondary structural changes and some rearrangements in the heme region. Moreover, ChCl:G seemed to protect VP from thermal denaturation probably by forming a protective layer around VP molecules. In contrast to experimental studies on hydrated ChCl:G, molecular dynamic (MD) simulations of VP in a box of pure ChCl:G suggested minor conformational changes. However, MD studies confirmed the rearrangements of the heme region in the presence of ChCl:G compared to water. According to the results, the authors concluded that lower concentration of ChCl:G could improve VP's activity and kinetic due to inducing some conformational changes, particularly in the heme vicinity, and also by stabilizing the intermediate complex during catalysis. |
| Databáze: | OpenAIRE |
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