Secretory and synthetic changes in the secretory proteinases of mouse submandibular glands after methoxamine stimulation, assessed histochemically by means of D-Val-Leu-Arg-methoxy-naphthylamide

Autor:	Masahiko Mori, Kouji Tsukitani, John R. Garrett
Rok vydání:	1993
Předmět:	Histology Salivary gland Physiology Stimulation Cell Biology Biology Biochemistry Submandibular gland Methoxamine Pathology and Forensic Medicine Staining Convoluted tubule medicine.anatomical_structure In vivo medicine Secretion medicine.drug
Zdroj:	ACTA HISTOCHEMICA ET CYTOCHEMICA. 26:51-55
ISSN:	1347-5800 0044-5991
DOI:	10.1267/ahc.26.51
Popis:	The secretion of proteinases and their resynthesis in the granular convoluted tubule (GCT) cells of mouse submandibular glands were studied after in vivo methoxamine (Methox) treatment (15mg/kg, i. p.), using a histochemical reaction with D-Val-Leu-Arg-4-methoxy-2-naphthylamide. The amidase reaction product was confined to the secretory granules in GCT cells, in unstimulated and stimulated glands. Methox caused extensive secretion of the granules. An increased staining reaction by the proteinases seemed to occur in granules prior to secretion, and fusions had often taken place. Reaccumulation of proteinases in the newly forming granules was found to start between 6 to 12hr after injection and the process was still incomplete at 48hr, but the granules showed less staining than at the earlier times. The variations observed in staining intensity may relate to the extent of binding with other proteins, and an unbinding of proteinases appears to be a possibility prior to the secretion of granules.
Databáze:	OpenAIRE
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