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The E. coli was selected among the bacteria having inhibitory effect described in the previous reports, and the experiments concerning the inhibitory effect of E. coli on the GDVII strain of mouse encephalomyeltisis virus (GDVII virus) were investigated in detail. The results obtained the following data: the administration with E. coli before virus inoculation was effective on the inhibition of the infection with the GDVII virus but when the E. coil was given to the mice following the virus infection the effect of the bacterium was not evident; it was most significant in the case of the same route of administration with the bacterium and the virus; the bacterium itself had not direct virucidal activity; the effect of the bacterium was not related with the antibody against this bacterium; and no evidence of inapparent infection in the survival mice was presented. Therefore it would be assumed that the inhibitory effect of E. coli on the virus infection might prevent the invasion or penetration of the virus into the susceptible cells.The purpose of this paper is to report a part of the kinetics of the inhibitory effect of E. coli on the virus infection by using the Maitland' method in which the suckling mice brains were employed as the explants. The results obtained demonstrated that the inhibitory effects of E. coli on the virus were significant when the tissues were cultured by the fluid containing the bacteria before the virus inoculatio. However, at the prolonging the culture time, it was indicated that the difference of virus multiplication between the experimental and the control group was small in the beginning of the culture, as the culture was getting later, the difference also lager. Therefore no evidence was presented the mechanism was either prevension of the virus-cell combination or convarsion of an intracellular process for virus multiplication. |
