The Interaction of DNA with Phenanthroline and New Phenanthrocyanine Complexes of Zn(II)
| Autor: | S. V. Paston, V. N. Demidov, A. O. Martynova, E. V. Akulenkova |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Quenching (fluorescence) Aqueous solution 030102 biochemistry & molecular biology Chemistry Phenanthroline Intercalation (chemistry) Biophysics Fluorescence spectroscopy Metal 03 medical and health sciences Nucleic acid thermodynamics chemistry.chemical_compound Crystallography 030104 developmental biology visual_art visual_art.visual_art_medium DNA |
| Zdroj: | Biophysics. 66:17-24 |
| ISSN: | 1555-6654 0006-3509 |
| DOI: | 10.1134/s0006350921010048 |
| Popis: | The interaction of DNA with complexes of Zn(II) and 1,10-phenanthroline derivatives in aqueous salt solutions has been studied by the optical spectroscopy, fluorescence, and DNA melting methods. Bis-(1,10-phenanthroline)-(1,10-phenanthrocyanine)-dizinc (II) acetate (diZn) and its precursor, bis-(1,10-phenanthroline)-zinc (II) acetate (monoZn) have been used. The interaction of DNA with phenanthroline significantly differs from that with phenanthroline metal complexes. MonoZn and diZn form complexes with DNA. Depending on the molar ratio of the components, ligands may interact with DNA in several ways; one of these is presumably intercalation. No saturation binding was detected at 0.1 ≤ r ≤ 20 and 0.1 ≤ r ≤ 1.67 for monoZn and diZn, respectively. The formation of secondary complexes of monoZn and diZn with DNA is possible. The interaction with DNA leads to quenching the fluorescence of the metal complexes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink