Evaluation of Hydrolytic Products of 17-Ketosteroids by Means of Gas-Liquid Chromatography

Autor:	Leo P. Cawley, Billy O. Musser, H. Learned, S. Beckloff, Wendell Faucette
Rok vydání:	1965
Předmět:	Etiocholanolone Chromatography Chemistry Biochemistry (medical) Clinical Biochemistry Ethyl acetate Urine Hydrolysis chemistry.chemical_compound Enzymatic hydrolysis medicine Degradation (geology) Organic chemistry Gas chromatography Perchloric acid medicine.drug
Zdroj:	Clinical Chemistry. 11:1009-1018
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/11.11.1009
Popis:	In the course of evaluating urinary 17-ketosteroid (17-KS) gas-liquid chromatographic patterns, a number of peaks with retention times shorter than that of 17-KS were constantly observed. This background material was found to be degradation products of 17-KS produced by HCI hydrolysis. A study with pure 17-KS as a mixture and as individual steroids was used to identify the steroids responsible for most of the 9 compounds with retention times less than etiocholanolone. Hydrolysis in ethyl acetate containing perchloric acid produced smaller amounts of degradation products than HCI-hydrolysis. Enzymatic hydrolysis appears to be the most acceptable system for preparation of the extracts from urine for this system of assay.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9d55ce209d8a1f9f4617d2883c4331a2 https://doi.org/10.1093/clinchem/11.11.1009 Zobrazit plný text záznamu