Separation of Mutagens from Drinking Water using Coupled Bioassay/Analytical Fractionation
| Autor: | John C. Loper, M. Wilson Tabor |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
endocrine system
Residue (complex analysis) Chromatography Chloroform Chemistry Health Toxicology and Mutagenesis fungi Extraction (chemistry) Public Health Environmental and Occupational Health food and beverages Soil Science Fractionation Reversed-phase chromatography Pollution High-performance liquid chromatography Analytical Chemistry Solvent chemistry.chemical_compound Environmental Chemistry Bioassay Waste Management and Disposal Water Science and Technology |
| Zdroj: | International Journal of Environmental Analytical Chemistry. 8:197-215 |
| ISSN: | 1029-0397 0306-7319 |
| DOI: | 10.1080/03067318008071500 |
| Popis: | A general preparative procedure has been developed for the isolation of mutagenic components from organic residues of drinking water. This procedure features the Salmonella-microsome mutagenicity assay coupled with an analytical fractionation method which progressively focuses to the bioactive constituents of the complex mixture. The three-step method consists of: 1) semi-solid/liquid extraction; 2) radially compressed column octadecyl-silane reverse phase HPLC; 3) SEP-PAKR concentration and solvent exchange of HPLC subfractions for chemical and biological characterization. Using as a model a carbon chloroform extracted organic residue mixture prepared from drinking water several years ago, three mutagenic HPLC subfractions were isolated, accounting for the bulk of the mutagenic activity of this complex mixture. Preliminary GC/MS results indicate the mutagenic activity is due to isomeric chlorinated aliphatic ethers. The biological characterization suggests the mutagenic specific activity of thes... |
| Databáze: | OpenAIRE |
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