Continuous biohydrogen production by a degenerated strain of Clostridium acetobutylicum ATCC 824
| Autor: | Karlo Guerrero, Iván Paredes, Raúl Conejeros, Roberto Gallardo, Julián Quintero, Silvia Mau, Juan Carlos Gentina, Germán Aroca |
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| Rok vydání: | 2021 |
| Předmět: |
Clostridium acetobutylicum
biology Strain (chemistry) Renewable Energy Sustainability and the Environment Chemistry Energy Engineering and Power Technology 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics biology.organism_classification 01 natural sciences Continuous production 0104 chemical sciences Butyric acid chemistry.chemical_compound Fuel Technology Yield (chemistry) Biohydrogen Steady state (chemistry) Food science 0210 nano-technology Hydrogen production |
| Zdroj: | International Journal of Hydrogen Energy. 46:5100-5111 |
| ISSN: | 0360-3199 |
| DOI: | 10.1016/j.ijhydene.2020.11.104 |
| Popis: | Degenerated strains of Clostridium acetobutylicum lack the ability to produce solvents and to sporulate, allowing the continuous production of hydrogen and organic acids. A degenerated strain of Clostridium acetobutylicum was obtained through successive batch cultures. Its kinetic characterization showed a similar specific growth rate than the wild type (0.25 h−1), a higher butyric acid production of 6.8 g·L−1 and no solvents production. A steady state was reached in a continuous culture at a dilution rate of 0.1 h−1, with a constant hydrogen production of 507 mL·h−1, corresponding to a volumetric rate of 6.10 L·L−1 d−1, and a yield of 2.39 mol of H2 per mole of glucose which represents 60% of the theoretical maximum yield. These results suggest that the degeneration is an interesting alternative for hydrogen production with this strain, obtaining a high hydrogen production in a continuous culture with cells in a permanent acidogenic state. |
| Databáze: | OpenAIRE |
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