Diversity, plant growth promoting and downy mildew disease suppression potential of cultivable endophytic fungal communities associated with pearl millet
| Autor: | Siddapura Ramachandrappa Niranjana, Sollepura B Rajini, M. Nandhini, H. S. Shetty, Ole Lund, A. C. Udayashankar, Harishchandra S. Prakash |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Fusarium biology food and beverages engineering.material biology.organism_classification 01 natural sciences Plant use of endophytic fungi in defense Crop 03 medical and health sciences 030104 developmental biology Insect Science Fusarium oxysporum Shoot Botany engineering Downy mildew Beneficial organism Agronomy and Crop Science Pearl 010606 plant biology & botany |
| Zdroj: | Biological Control. 127:127-138 |
| ISSN: | 1049-9644 |
| DOI: | 10.1016/j.biocontrol.2018.08.019 |
| Popis: | In the present study, endophytic fungi were isolated from different parts of pearl millet crop from different regions of Karnataka. Endophytes were screened for diversity in tissue types, plant growth promoting (PGP) traits and downy mildew disease suppression ability in pearl millet. A total of 202 fungal isolates were isolated from leaves, shoot and root regions. The diversity analysis revealed that the plant harbored diverse fungal groups and the dominant fungi varied according to the sampling regions. The isolates were assigned to 29 fungal species based on morphology and molecular characterization using ITS sequence. All the isolates belonged to the phylum Ascomycota. Fusarium species were the most dominant fungi. Out of 29 representative isolates tested for plant growth promoting traits in vitro and in vivo, seven isolates were observed to improve the growth of pearl millet in comparison to control. Among the seven isolates, Fusarium oxysporum, Trichoderma asperellum and Acremonium sp. were found to suppress the downy mildew disease incidence by 36% in greenhouse conditions. The potent isolates were confirmed to behave as endophytes using pathogenicity, SEM and confocal microscopy experiments. This paper underlines the value of the endophytes as untapped suitable source of beneficial microorganisms that can be used to control pathogens in a sustainable way. |
| Databáze: | OpenAIRE |
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