| Popis: |
prepare 100 mL per 250 mL flask for each overnight culture for CZV8CS - liquid media for flask culture prepare 100 mL per 250 mL flask for each overnight culture for 100 mL: 300 mL Czapek-Dox liquid medium 4.54 g 13.62 g centrifuged V8 juice 20 mL 60 mL add H20 to 95 mL 285 mL adjust to pH 6.0 transfer to transfer to flask(s) and autoclave add 5 mL sterile complete supplement to media after autoclaving Complete Supplement (CS) Bacto-casamino acids 20 g/l Bacto-peptone 20 g/l Bacto-yeast extract 20g/l Adenine 3 g/l 9 Biotin 0.02 g/l Nicotinic acid 0.02 g/l P-aminobenzoic acid 0.02 g/l 10 Pyridoxine 0.02 g/l Thiamine 0.02 g/l |
