The impact of pre-existing B-cell memory in the response to Influenza vaccination
| Autor: | Rodrigo B. Abreu, Greg A. Kirchenbaum, Emily F. Clutter, Giuseppe Andrea Sautto, Anne-Gaelle B. Blackwell, Ted M. Ross |
|---|---|
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 202:139.19-139.19 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.202.supp.139.19 |
| Popis: | Influenza is a highly contagious viral respiratory disease with more than 200 thousand cases reported in the US last season (2017/18). Annual vaccination is recommended by the World Health Organization with the goal to reduce influenza severity and limit transmission. However, currently available split inactivated influenza vaccines can only elicit protection up to ~60% in well-matched-strain years. Furthermore, vaccine effectiveness frequently varies between different influenza subtypes within a single influenza season for unclear reasons. Immunological imprinting from early-life influenza infection can prominently shape the immune response to subsequent infections. Here, the impact of pre-existing B-cell memory in the response to quadrivalent influenza vaccine was assessed. Blood samples were collected from healthy subjects (18 to 85 years old) prior to vaccination and 21–28 days after vaccination with quadrivalent influenza vaccine. Samples were assessed for changes in serological antibodies to the hemagglutinin protein (HA) by ELISA and by hemagglutination-inhibition (HAI) assays against the four strains in the vaccine. The number of antigen specific memory B-cells (Bmem) were quantified by flow cytometry and the polyclonal Bmemresponse was assessed against the HA of the four vaccine strains. Influenza vaccination greatly induces the HA-specific Bmem-cell pool and, despite no differences in antigenicity between the four vaccine components, most individuals are biased towards one of the influenza A or Influenza B vaccine strains. Overall, this study unveils a new mechanism behind the differences in vaccine effectiveness against different influenza subtypes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|