Studying Protein and Gold Nanoparticle Interaction Using Organothiols as Molecular Probes
| Autor: | Leyuan Yu, Dong Liu, Karthikeshwar Vangala, Dongmao Zhang, Fathimar Ameer, Edwin A. Lewis, George Salomon, Vu H. Le |
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| Rok vydání: | 2012 |
| Předmět: |
biology
Chemistry Kinetics Analytical chemistry Nanoparticle engineering.material Surfaces Coatings and Films Electronic Optical and Magnetic Materials General Energy Adsorption Coating Chemical engineering Monolayer biology.protein engineering Physical and Theoretical Chemistry Bovine serum albumin Molecular probe Layer (electronics) |
| Zdroj: | The Journal of Physical Chemistry C. 116:3645-3652 |
| ISSN: | 1932-7455 1932-7447 |
| DOI: | 10.1021/jp2107318 |
| Popis: | The protein and gold nanoparticle (AuNP) interfacial interaction has broad implications for biological and biomedical applications of AuNPs. In situ characterization of the morphology and structural evolution of protein on AuNPs is difficult. We have found that the protein coating layer formed by bovine serum albumin (BSA) on AuNP is highly permeable to further organothiol adsorption. Using mercaptobenzimidazole (MBI) as a molecular probe, it is found that BSA interaction with AuNP is an exceedingly lengthy process. Structural modification of BSA coating layer on AuNP continues even after 2 days’ aging of the (AuNP/BSA) mixture. While BSA is in a near full monolayer packing on the AuNPs, it passivates only up to 30% of the AuNP surfaces against MBI adsorption. Aging reduces the kinetics of the MBI adsorption. However, even in the most aged BSA-coated AuNP (3 days), 80% of the MBI adsorption occurs within the first 5 min of the MBI addition to the (AuNP/BSA) mixture. The possibility of MBI displacing the a... |
| Databáze: | OpenAIRE |
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