| Autor: |
Toshiki Yasokawa, Hiroaki Kobayashi, Ryoji Hyodo, Yasutake Inoue, Fumikazu Oohira, Katsumi Ishizaki, Ichirou Ishimaru, Akihiro Hayashi |
| Rok vydání: |
2004 |
| Předmět: |
|
| Zdroj: |
Optomechatronic Micro/Nano Components, Devices, and Systems. |
| ISSN: |
0277-786X |
| DOI: |
10.1117/12.571134 |
| Popis: |
Currently, diagnosis of cancer is performed by biopsy, whereby medical doctors observe a removed specimen, focusing their attention on morphological changes in the cell sequence and cell nuclei. For early cancer, the only effect is a slight increase in the size of the cell nuclei in comparison with normal cells. Based on medical knowledge, it is presumed that an extremely small amount of a specific protein may be contained in a cell nucleus. We propose spectroscopy-tomography of single cell to measure slight changes in this protein. This technology is composed of two elemental technologies, high spatial resolution spectrometry and a precise single cell rotating method. We propose variable phase-contrast spectrometry as the high spatial resolution spectrometry and proximal two-beam optical tweezers as the precise rotating method. By these methods, we can obtain a 3-dimensional distribution of the cell components to a high spatial resolution. We verified the accuracy of variable phase-contrast spectrometry by measuring the height of a diffraction grating. We confirmed that a microsphere can be rotated by proximal two-beam optical tweezers. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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