PARP-1 regulates pro-inflammatory cell death (34.13)
| Autor: | RK Subbarao Malireddi, Thirumala-Devi Kanneganti |
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| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 184:34.13-34.13 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.184.supp.34.13 |
| Popis: | PARP-1(poly (ADP-ribose) polymerase 1), an enzyme involved in DNA repair, is a nuclear chromatin associated multifunctional protein and belongs to a large family of enzymes that can synthesize long branched polymers of ADP-ribose units by using NAD+( Nicotinamide adenine dinucleotide) as the substrate. DNA strand nicks and breaks activate PARP-1 and the activated enzyme makes branched chains of up to 200 ADP-ribose units and adds covalently to various nuclear proteins such as histones and especially PARP-1 itself. PARP-1 has been shown to play a major role in a number of pathophysiological situations. In this study, we show that cytokine and chemokine profiles from PARP-WT and -KO BMDM showed no significant differences. However, IL1-β secretion is reduced in PARP-1 deficient macrophages, suggesting a role for PARP-1 in pro-inflammatory signaling. PARP-1 deficient macrophages had displayed prominent protection from pro-inflammatory cell death in response to LPS+ATP stimuli. Together, these results demonstrate that PARP-1 contribute to the pathophysiology by regulating the pro-inflammatory cytokine profiles. |
| Databáze: | OpenAIRE |
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