| Autor: |
Thamm, Joseph P. McCain, Faquin Wc, Guastaldi Fps, Mohamed A. Hakim, Lowe B, A. Liapaki |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Journal of Dentistry & Oral Disorders. 7 |
| ISSN: |
2572-7710 |
| DOI: |
10.26420/jdentoraldisord.2021.1159 |
| Popis: |
Temporomandibular Joint (TMJ) disorders affect up to 10-40% of the population and if left untreated, may eventually lead to Osteoarthritis (OA) of the TMJ. In vivo TMJ repair and regeneration has received significant attention and represents a promising approach for the treatment of degenerative TMJ disorders. The aim of this study was to present a pre-clinical mouse model of TMJ articular cartilage defect and evaluate the utility of a potential tissue engineered TMJ therapy utilizing Mesenchymal Stem Cells (MSCs) derived from mice condyle, hydrogel, and biosilica. C57BL/6 mice (n=30) were equally divided into the following groups: sham group (S-group); control group, condylar cartilage defect only (CD-group); experimental group, condylar cartilage defect + direct administration of MSCs+hydrogel+biosilica (H-group). Mice were euthanized at 4 (n=15) and 8 (n=15) weeks and TMJ joint specimens were harvested for analysis. H&E and Safranin O stained sections showed intact articular surfaces on the condyle and glenoid fossa at both time points, maturation and distribution of chondrocytes along the condyle for the H-group compared to the CD-group. Data from this preliminary study shows that MSCs+hydrogel+biosilica may represent an experimental therapeutic compound for TMJ condylar cartilage regeneration. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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