A Fast and Sensitive Coomassie Blue Staining for Proteins in Polyacryl-Amide Gels using Ion-Pairing Agent
| Autor: | Gyurng-Soo Yoo, Hee-Youn Hong, Sang-Hyoun Yoon, Dong-Ki Choi, Tae-Ik Kwon, Do-Seong Na, Jung-Kap Choi |
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| Rok vydání: | 1996 |
| Předmět: |
Detection limit
Gel electrophoresis Chromatography biology Chemistry Coomassie Brilliant Blue Biochemistry (medical) Clinical Biochemistry Polyacrylamide Biochemistry Analytical Chemistry Staining chemistry.chemical_compound Electrochemistry biology.protein Tetraoctylammonium bromide Bovine serum albumin Sodium dodecyl sulfate Spectroscopy |
| Zdroj: | Analytical Letters. 29:1517-1525 |
| ISSN: | 1532-236X 0003-2719 |
| DOI: | 10.1080/00032719608001501 |
| Popis: | Here we describe a fast and sensitive Coomassie blue staining method for detection of proteins in polyacrylamide gels using an ion-pairing agent, tetraoctylammonium bromide (TOA). To increase sensitivity, sodium dodecyl sulfate (SDS) in an electrophoresed gel was removed by washing with water for 5 min, and then the gel was stained in the mixed solution of 0.25% Coomassie brilliant blue R-250 (CB), and 1% TOA (mixed up at a 10 : 0.6 volume ratio). TOA inhibits the binding of CB to gel matrix by forming an ion-pair complex, thus not only increases the staining of CB but also helps make background destaining easy. This staining method reduces the staining and destaining times to 50 min and increases the detection limit up to 25 ng of bovine serum albumin (BSA). |
| Databáze: | OpenAIRE |
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