Analysis of Aldehyde-bisulfites in Beer by HPLC-Fluorescence Detection with Post-column Derivatization

Autor:	Masachika Takashio, Teruo Tamaki, Toshihiko Osawa, Shouhei Koshino, Hirotaka Kaneda, Shunro Kawakishi
Rok vydání:	1996
Předmět:	Detection limit chemistry.chemical_compound Chromatography chemistry Sulfite Acetaldehyde Formaldehyde Disulfite High-performance liquid chromatography Ammonium acetate Fluorescence spectroscopy Food Science
Zdroj:	Journal of Food Science. 61:105-108
ISSN:	1750-3841 0022-1147
DOI:	10.1111/j.1365-2621.1996.tb14736.x
Popis:	An analytical method was based on the aldehyde-bisulfite (separated by HPLC) reaction with o-phthalaldehyde in the presence of ammonium acetate, producing a fluorescent compound. Detection limits of alkanal-bisulfite (from formaldehyde- to octylaldehyde-bisulfite) and 2-alkenal-bisulfite (from trans-2-hexenal- to trans-2-nonenal-bisulfite) ranged from 5 nM to 25 nM. The method was less sensitive for free sulfite (detection limit 100 μM) than for aldehyde-bisulfite. Increasing acetaldehyde-bisulfite with addition of 25 mM acetaldehyde to a beer sample showed free sulfite in the beer. Free sulfite and acetaldehyde-bisulfite were detected in commercial beers but other aldehyde-bisulfites were not.
Databáze:	OpenAIRE
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