Biolistic transformation ofcry 1Ac gene in eggplant (Solanum melongenaL.)

Autor:	J. S. Sandhu, Ajmer Singh Dhatt, S. S. Gosal, M. K. Sidhu
Rok vydání:	2014
Předmět:	food.ingredient Spots biology fungi food and beverages biology.organism_classification Transformation (genetics) Horticulture food Cry1Ac Shoot Botany Solanum Gene Cotyledon Explant culture
Zdroj:	International Journal of Agriculture, Environment and Biotechnology. 7:679
ISSN:	2230-732X 0974-1712
DOI:	10.5958/2230-732x.2014.01375.8
Popis:	Among different explants, cotyledon showed highest regeneration and number of buds per explant on all media combinations. However, highest regeneration potential was observed on MS fortified with 3.0 mgl−1 BAP (80.36%), but number of buds per explant were highest (21.76) on MS media supplemented with 2.5 mgl−1 BAP+ 1.0 mgl−1 Kin. Plant regeneration of cotyledons was completely checked with a selection pressure of 15ppm to 25ppm hygromycin for untranformed explants. But the selection efficacy of transformed tissue was 19.56% with 20ppm and 66.66% with 15 ppm hygromycin. GUS gene was expressed in 76.92% bombarded explants with 2 to 7 GUS spots per explant confirming the presence of transformed tissue. GUS gene was also visualized in 66.66% regenerated shoots from bombarded tissue. PCR analysis of putative transgenic eggplant plants with the specific primers confirmed the presence of Cry1Ac gene with 1.2 KB product size (falling between 1KB and 2KB bands of 10KB DNA ladder) in two of the four plants.
Databáze:	OpenAIRE
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