Overexpression of jasmonic acid carboxyl methyltransferase increases tuber yield and size in transgenic potato

Autor: Jeong-Han Kim, Ju Seok Seo, Jong-Joo Cheong, Jong Seob Lee, Yang Do Choi, Han Yong Lee, Hwang Bae Sohn, Choonkyun Jung, Yin Won Lee, Jae Heung Jeon
Rok vydání: 2010
Předmět:
Zdroj: Plant Biotechnology Reports. 5:27-34
ISSN: 1863-5474
1863-5466
Popis: Jasmonates control diverse plant developmental processes, such as seed germination, flower, fruit and seed development, senescence and tuberization in potato. To understand the role of methyl jasmonate (MeJA) in potato tuberization, the ArabidopsisJMT gene encoding jasmonic acid carboxyl methyltransferase was constitutively overexpressed in transgenic potato plants. Increases in tuber yield and size as well as in vitro tuberization frequency were observed in transgenic plants. These were correlated with JMT mRNA level––the higher expression level, the higher the tuber yield and size. The levels of jasmonic acid (JA), MeJA and tuberonic acid (TA) were also higher than those in control plants. Transgenic plants also exhibited higher expression of jasmonate-responsive genes such as those for allene oxide cyclase (AOC) and proteinase inhibitor II (PINII). These results indicate that JMT overexpression induces jasmonate biosynthesis genes and thus JA and TA pools in transgenic potatoes. This results in enhanced tuber yield and size in transgenic potato plants.
Databáze: OpenAIRE
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