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Background and aims Soil microorganisms play key roles in the cyclings of soil nutrient and ecosystem stability, however, the soil microbial community composition often is influenced by nutrient enrichment. Increased nitrogen (N) input endangers plant species diversity and stability of grassland ecosystems, and which should be often affected by the addition of phosphorus (P) addition. However, the resistance of soil microbial community to N and P additions, and their interactions in the N-limited temperate meadow ecosystem remains poorly understood.Methods We carried out a seven-year experiment with the additions of N and P in a temperate meadow. The community structures of soil bacteria and fungi were examined based on high-throughput sequencing targeting the 16S rRNA and ITS genes, respectively.Results N addition did not influence on the community composition and species richness of bacteria, while altered soil fungal community composition and increased fungal OTU richness. P addition significantly altered soil fungal and bacterial community compositions and declined the richness of bacterial OTU, increased the OTU richness of fungi. The Proteobacteria (38.5%) and Acidobacteria (22.3%) were the most dominant bacteria. The Ascomycota was the dominant fungi (42.6%) across all samples. The enrichment of soil available P concentration caused by the addition of P played key roles in the bacterial β-diversity, while the β-diversity of soil fungi was mainly influenced by the concentrations of soil N and P, and soil moisture. Conclusions The sensitivity of soil fungi and bacteria to the addition of P is stronger than N addition, and which is more sensitive than the plant community. Our result highlights the unequal sensitivity of soil fungal and bacterial community composition and structure to the eutrophications of N and P, thereby causing changes in above and belowground communities and influencing ecosystem stability in the studied temperate meadow ecosystem. |
