Analysis of plasma free fatty acid cyanomethyl derivatives by GC-NPD for the diagnosis of mitochondrial fatty acid oxidation disorders
| Autor: | H. S. Shin, M. J. Paik, Piero Rinaldo, C. Yu, H. R. Yoon |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
chemistry.chemical_classification
Detection limit Nitrogen–phosphorus detector Chromatography Organic Chemistry Clinical Biochemistry Fatty acid Biochemistry Analytical Chemistry chemistry.chemical_compound chemistry Blood plasma lipids (amino acids peptides and proteins) Gas chromatography Derivatization Quantitative analysis (chemistry) Beta oxidation |
| Zdroj: | Chromatographia. 52:211-216 |
| ISSN: | 1612-1112 0009-5893 |
| Popis: | Early diagnosis of fatty acid oxidation (FAO) disorders is important to reduce severe morbidity and mortality. Although analysis of plasma free fatty acids (FFAs) is frequently performed using stable isotope-dilution gas chromatography-mass-spectrometry (GC-MS), there are institutions where the required instrumentation is not available to support a rapid work-up of acutely ill patients. For this reason, we have developed a novel cyanomethyl derivatization method for FFAs which is followed by GC analysis of the resulting esters using nitrogen-phosphorus detector (NPD) for the rapid diagnosis of mitochondrial fatty acid oxidation disorders. FFAs were extracted from plasma and derivatized to the cyanomethyl ester by heating with bromoacetonitrile at 60°C for 30 min GC-NPD analysis was then performed. The mean recoveries of C6:0-C18:0FFAs were between 87% abd 96%. The method detection limits (S/N=3) were 0.1–0.5 ng for C6:0-C14:0 FFAs, and 0.001–0.01 ng for C16:0-C18:0 FFAs. We succesfully performed differential diagnosis of representative FAO disorders from the confimed patient's plasmas. This simple method offers cost-effective and time-saving alternative to GC-MS for the biochemical diagnosis of selected FAO disorders. |
| Databáze: | OpenAIRE |
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