Reverse depletion effects and the determination of ligand density on some spherical bioparticles
| Autor: | Yanhui Liu, Yijun Liu, Yangtao Fan, Qiancheng Li, Chunxiang Wang, Hou-Qiang Xu |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Ligand Chemistry General Physics and Astronomy Nanotechnology 02 engineering and technology 021001 nanoscience & nanotechnology Endocytosis Chromatin 03 medical and health sciences 030104 developmental biology Microtubule Quantum dot Biophysics Chromatin Loop 0210 nano-technology Cytoskeleton Macromolecule |
| Zdroj: | Journal of Experimental and Theoretical Physics. 122:1117-1121 |
| ISSN: | 1090-6509 1063-7761 |
| DOI: | 10.1134/s1063776116050228 |
| Popis: | In cell environments crowded with macromolecules, the depletion effects act and assist in the assembly of a wide range of cellular structures, from the cytoskeleton to the chromatin loop, which are well accepted. But a recent quantum dot experiment indicated that the dimensions of the receptor–ligand complex have strong effects on the size-dependent exclusion of proteins in cell environments. In this article, a continuum elastic model is constructed to resolve the competition between the dimension of the receptor–ligand complex and depletion effects in the endocytosis of a spherical virus-like bioparticle. Our results show that the depletion effects do not always assist endocytosis of a spherical virus-like bioparticle; while the dimension of the ligand–receptor complex is larger than the size of a small bioparticle in cell environments, the depletion effects do not work and reverse effects appear. The ligand density covered on the virus can be identified quantitatively. |
| Databáze: | OpenAIRE |
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