Hedgehog signaling pathway regulated the target genes for adipogenesis in silkwormBombyx mori
| Autor: | Rui-ting Chen, Yan Lu, Hu-hu Xin, Mei-xian Wang, Shuang Liang, Deng-pan Zhang, Yun-gen Miao |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Gene knockdown Cyclopamine fungi Adipose tissue Biology General Biochemistry Genetics and Molecular Biology Hedgehog signaling pathway Cell biology chemistry.chemical_compound Endocrinology chemistry Adipogenesis Insect Science Adipocyte Internal medicine medicine Signal transduction Agronomy and Crop Science Hedgehog Ecology Evolution Behavior and Systematics |
| Zdroj: | Insect Science. 22:587-596 |
| ISSN: | 1672-9609 |
| DOI: | 10.1111/1744-7917.12164 |
| Popis: | Hedgehog (Hh) signals regulate invertebrate and vertebrate development, yet the role of the pathway in adipose development remains poorly understood. In this report, we found that Hh pathway components are expressed in the fat body of silkworm larvae. Functional analysis of these components in a BmN cell line model revealed that activation of the Hh gene stimulated transcription of Hh pathway components, but inhibited the expression of the adipose marker gene AP2. Conversely, specific RNA interference-mediated knockdown of Hh resulted in increased AP2 expression. This further showed the regulation of Hh signal on the adipose marker gene. In silkworm larval models, enhanced adipocyte differentiation and an increase in adipocyte cell size were observed in silkworms that had been treated with a specific Hh signaling pathway antagonist, cyclopamine. The fat-body-specific Hh blockade tests were consistent with Hh signaling inhibiting silkworm adipogenesis. Our results indicate that the role of Hh signaling in inhibiting fat formation is conserved in vertebrates and invertebrates. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text