Interferon-induced proteins. Purification and characterization of a 15,000-dalton protein from human and bovine cells induced by interferon

Autor:	E. Knight, G J Jonak, D C Blomstrom, B D Korant
Rok vydání:	1984
Předmět:	Messenger RNA RNA Cell Biology Biology Biochemistry Molecular biology In vitro Interferon Cell culture Protein purification Protein A/G biology.protein medicine Protein biosynthesis Molecular Biology medicine.drug
Zdroj:	Journal of Biological Chemistry. 259:14835-14839
ISSN:	0021-9258
DOI:	10.1016/s0021-9258(17)42679-2
Popis:	Human interferons induce a protein of 15,000 daltons in human and bovine cells. This protein is located in the cytoplasm in a soluble form and is induced by concentrations of interferon which induce the antiviral state. Messenger RNA prepared from interferon-treated human and bovine cells contains a mRNA which yields on translation in vitro a protein similar in size to the 15-kDa protein induced by interferon in vivo. The human protein has been purified to homogeneity from interferon-treated human cells by ion-exchange chromatography and reverse-phase high-performance liquid chromatography. A comparison of the peptides generated by V8 protease from the human and bovine 15-kDa proteins reveals that the two proteins are similar but not identical.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9c8ff288ad4b5e1fa2c9ee6184f16fc8 https://doi.org/10.1016/s0021-9258(17)42679-2 Zobrazit plný text záznamu