Tandem Mass Spectrometry Using an Axially Resonant Excitation Linear Ion Trap

Autor:	Takashi Baba, Izumi Waki, Hiroyuki Satake, Masuyuki Sugiyama, Yuichiro Hashimoto, Hideki Hasegawa
Rok vydání:	2007
Předmět:	Electron-capture dissociation Physics::Plasma Physics Chemistry Direct current Analytical chemistry Ion trap Atomic physics Quadrupole ion trap Tandem mass spectrometry Dissociation (chemistry) Excitation Ion
Zdroj:	Journal of the Mass Spectrometry Society of Japan. 55:339-342
ISSN:	1880-4225 1340-8097
DOI:	10.5702/massspec.55.339
Popis:	We performed collision-induced dissociation (CID) and electron capture dissociation (ECD) analyses using a new ion trap: an axially resonant excitation linear ion trap (AREX LIT). Unlike a conventional linear ion trap, the AREX LIT can trap and detect low m/z fragment ions, such as immonium ions and iTRAQTM reporter ions, that are produced by CID. This capability to detect low m/z fragments is realized by CID excitation in a nearly harmonic direct current potential along the linear axis. In contrast, CID excitation in a conventional linear ion trap is in a pseudo-harmonic radio frequency potential that destabilizes fragment ions whose m/z is less than 1/4 that of the precursor ions. ECD was achieved in an axial magnetic field of about 0.2 Tesla superimposed along the AREX LIT. We observed that sequential ECD/CID inside the trap improved the sequence coverage of a peptide.
Databáze:	OpenAIRE
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