Metabolic engineering ofEscherichia colifor production of non-natural acetins from glycerol
| Autor: | Hogyun Seo, Seongjoon Joo, Jung-Hoon Sohn, Kyung-Jin Kim, Tenzin Tseten, Chonglong Wang, Bakht Zada, Seon-Won Kim, Seong-Hee Jeong |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
chemistry.chemical_classification 0303 health sciences Substrate (chemistry) Maltose medicine.disease_cause 01 natural sciences Pollution Chemical synthesis Metabolic engineering 03 medical and health sciences chemistry.chemical_compound Enzyme chemistry Biochemistry 010608 biotechnology medicine Glycerol Environmental Chemistry Escherichia coli Triacetin 030304 developmental biology |
| Zdroj: | Green Chemistry. 22:7788-7802 |
| ISSN: | 1463-9270 1463-9262 |
| DOI: | 10.1039/d0gc02395g |
| Popis: | Mono-, di- and triacetin are three glycerol esters which are usually synthesized via costly and environmentally unfriendly chemical synthesis methods. Here, Escherichia coli is metabolically engineered for the production of mono-, di- and triacetin using glycerol as a substrate. First, a novel biosynthetic route of mono- and diacetin is established by overexpression of a native enzyme, maltose O-acetyltransferase (MAA). Next, the biosynthetic pathway is extended to produce a mixture of mono-, di- and triacetin by overexpression of chloramphenicol-O-acetyltransferase (CAT). By successive strategies, including heterologous gene expression, metabolic engineering, and culture optimization, a recombinant E. coli is enabled to produce more than 27 g L−1 of a mixture of mono-, di- and triacetin in shake flask cultures, which is a >650-fold increase over the initial production of 0.04 g L−1. In vitro studies confirm the acetylation of glycerol to mono- and diacetin by MAA, and the additional acetylation to triacetin by CAT. When crude glycerol is used as a substrate, the engineered strain produced a total of 25.9 g L−1 of the acetin mixture, about the same as that achieved from pure glycerol. To our knowledge, this is the first successful report of microbial production of the artificial chemicals, acetins. |
| Databáze: | OpenAIRE |
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