Determination of chlorothalonil in water and agricultural products by a magnetic particle-based enzyme immunoassay

Autor:	Adrian M. Gueco, Scott W. Jourdan, James R. Fleeker, David P. Herzog, Fernando M. Rubio, Adele M. Scutellaro, Timothy S. Lawruk
Rok vydání:	1995
Předmět:	Detection limit Chromatography medicine.diagnostic_test Chlorothalonil Parts-per notation General Chemistry Pesticide chemistry.chemical_compound chemistry Immunoassay Acetone medicine Gas chromatography General Agricultural and Biological Sciences Surface water
Zdroj:	Journal of Agricultural and Food Chemistry. 43:1413-1419
ISSN:	1520-5118 0021-8561
DOI:	10.1021/jf00053a052
Popis:	A competitive enzyme immunoassay for the quantitation of chlorothalonil in water and agricultural products was developed. This immunoassay utililizes amine-terminated superparamagnetic particles as the solid phase to covalently attach polyclonal rabbit anti-chlorothalonil antisera. Specificity studies indicate that the antibody can distinguish chlorothalonil from its major metabolites and structurally similar pesticides. The immunoassay has a detection limit of 0.07 parts per billion (ppb, ng/mL) in water. The assay compares favorably with GC measurements when water samples are analyzed (r = 0.984). Studies indicate that the assay can potentially be used to analyze agricultural products after homogenization with acetone (detection limit of 20 ppb for celery) or to analyze dislodgable residues after a methanol surface wash (detection limit of 5 ppb for snow pea pods).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9c6681363a891b5e88fa26d64e01921e https://doi.org/10.1021/jf00053a052 Zobrazit plný text záznamu