Fluorescent detection of uric acid in biological samples through the inhibition of cobalt(II) catalyzed Amplex UltraRed
| Autor: | Chang Wei Huang, Mei Yao Wu, Chieh Yi Wang, Yang-Wei Lin, Tzu Ting Wei |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Radical
chemistry.chemical_element 02 engineering and technology 01 natural sciences chemistry.chemical_compound Allantoin Materials Chemistry Electrical and Electronic Engineering Instrumentation Detection limit Chromatography 010401 analytical chemistry Metals and Alloys 021001 nanoscience & nanotechnology Condensed Matter Physics Fluorescence 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials chemistry Reagent Urea Uric acid 0210 nano-technology Cobalt Nuclear chemistry |
| Zdroj: | Sensors and Actuators B: Chemical. 244:357-364 |
| ISSN: | 0925-4005 |
| Popis: | A novel fluorescent probe consisting of cobalt(II)/hydrogen peroxide–Amplex ® UltraRed (Co 2+ /H 2 O 2 –AUR) for determining uric acid (UA) concentration is reported. In the presence of H 2 O 2 , Co 2+ ions underwent oxidation to produce Co 3+ ions and hydroxyl radicals, which then reacted with the fluorescence reagent, AUR, forming a fluorescence product at a pH of 9.0. If UA was present, it underwent oxidation to form urea, parabanic acid, and allantoin. This inhibited the Co 2+ ions-catalyzed oxidation of AUR and resulted in a substantial decreasing in fluorescence intensity that was dependent on UA concentration. The buffer systems, pH values, and the concentrations of Co 2+ ions, AUR, and H 2 O 2 were crucial parameters in determining the sensitivity and selectivity of the method for sensing UA. Under optimal conditions, the fluorescence intensity decreased linearly with UA concentration in the 0.05–1.0 μmol L −1 range (R 2 = 0.94) with a detection limit of 20 nmol L −1 at a signal-to-noise ratio of 3. The analytical recoveries in urine and human serum samples were 90%–100.7% and 90%–95% respectively. This method is simpler and more cost effective than are other optical methods for the detection of UA in biological samples. |
| Databáze: | OpenAIRE |
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