In-Lab Manufacturing of Decellularized Rat Renal Scaffold for Kidney Bioengineering
| Autor: | Andrea Peloso, Lorenzo Cobianchi, Szandra Brambilla, Valeria Corradetti, Graziano Oldani, Marcello Maestri, Francesco Calabrese, Tommaso Dominioni, Antonio Citro |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Kidney Scaffold Decellularization Chemistry 02 engineering and technology 021001 nanoscience & nanotechnology medicine.disease Regenerative medicine Extracellular matrix Transplantation 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Biological property medicine 0210 nano-technology Kidney transplantation Biomedical engineering |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493976553 |
| Popis: | Whole-organ decellularization is recently gaining interest in the transplantation field as strategy to obtain acellular scaffold only composed by extracellular matrix. These structures, that still remain organ-specific in terms of biological cues and tridimensional morphology could be then recellularized with patient's autologous cells. The final result should be a nwe transplantable autologous organ that should by-pass, at the same time, the problem of organ shortage and secondly the consequences related to the immunosuppression need. Herein we describe the protocol to manufacture a whole-organ transplantable rat kidney scaffold by a dual-detergent (Triton X-100 and SDS) arterial peristaltic perfusion. Final results show whole-renal acellular scaffold with contextual preservation of tridimensional architecture and biological properties deriving from the extracellular matrix composition. |
| Databáze: | OpenAIRE |
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