Involvement of Prostaglandins in the Pharmacological Actions of L‐Cysteine in Ocular Tissues

Autor: Ya Fatou Njie-Mbye, Jenaye Robinson, Olivia Nguyen, Leah Mitchell, Sunny E. Ohia, Catherine A. Opere
Rok vydání: 2015
Předmět:
Zdroj: The FASEB Journal. 29
ISSN: 1530-6860
0892-6638
Popis: Evidence from our laboratory demonstrates that hydrogen sulfide (H2S) can exert pharmacological actions in ocular tissues, an effect that can be blocked by inhibitors of cyclooxygenase (COX). Purpose: To investigate whether H2S (using L-cysteine as substrate) can alter prostaglandin E2 (PGE2) production in isolated bovine irides and retina. Methods: Isolated bovine irides and retinae were incubated in oxygenated Krebs buffer for 30 mins, prior to treatment with different concentrations of L-cysteine (1 nM - 10 µM) and norepinephrine (NE, 1 µM). After incubation and termination of reaction, tissues were homogenized and both homogenates and incubation media were collected and prepared for PGE2 Enzyme-immunoassay (EIA) using a well-established methodology. Results: In both bovine isolated irides and retinae, L-cysteine elicited a concentration-dependent (1 nM - 10 µM) increase in PGE2 concentrations over basal levels. For instance, L-cysteine (10nM; irides) and (10 µM; retina) produced an equi-effective sign...
Databáze: OpenAIRE
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