| Autor: |
Mélanie Pellisson, Lucy Kirchhofer-Allan, Guglielmo Roma, Matthias Mueller, Sven Schuierer, Anne-Marie Zeeman, Erika L. Flannery, Matthias Rottmann, Sebastian A. Mikolajczak, Thierry Doll, Clemens H. M. Kocken, Pascal Maeser |
| Rok vydání: |
2021 |
| Předmět: |
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| Popis: |
Plasmodium cynomolgi (Pc) is one of the few parasite species that forms quiescent liver stage parasites known as hypnozoites and is therefore a suitable model for Plasmodium vivax. Very little is known about liver stage dormancy, which hampers the search for compounds with anti-hypnozoite activity. Here, we present the development of a Pc in vitro infection model using stem cell-derived hepatocytes from Macaca fascicularis. IPS cells were established on feeder free condition and differentiated into hepatocytes via inducible overexpression of key transcription factors. The generated hepatocytes were infected with Pc sporozoites and hypnozoite formation as well as schizont development were confirmed by immunofluorescence. This system is a promising tool to study the mechanisms underlying liver stage dormancy and facilitate drug discovery against hypnozoites. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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