| Popis: |
Guard cell protoplasts (GCP’s) were first studied by Zeiger and Hepler (1976), although Davey et al. (1974) had earlier experimented with isolated epidermal cell protoplasts from tobacco leaves. The microchamber method pioneered by Zeiger and Hepler allowed the observation of small numbers of GCP’s adhering to the remnants of strips of leaf epidermis, but did not allow a full range of microbiological techniques to be employed (Zeiger 1981). The full potential of the protoplast technique could only be realised when methods were developed for obtaining high yields of GCP’s and for their purification (Schnabl et al. 1978, Outlaw et al. 1981, Fitzsimons and Weyers 1983). |
