An Astrocyte Structural Organization Analysis Based on Fluorescent Microscopy with 2D and 3D Quantitative Approaches

Autor:	O. I. Nosova, D. A. Sufieva, D. E. Korzhevsky
Rok vydání:	2021
Předmět:	0301 basic medicine Structural organization 030102 biochemistry & molecular biology Structure analysis Confocal Cell Biology Biology 03 medical and health sciences 030104 developmental biology Neuronal homeostasis medicine.anatomical_structure Fluorescence microscope medicine Neuroscience Astrocyte
Zdroj:	Cell and Tissue Biology. 15:273-279
ISSN:	1990-5203 1990-519X
DOI:	10.1134/s1990519x21030081
Popis:	According to the obtained data over the last decades, astrocytes play a crucial role in neuronal homeostasis. Therefore, the software for assessing morphological changes in astrocytes is not sufficiently covered in the literature. Hence, this article examines a two- and three-dimensional quantitative approach to astrocyte structure analysis based on freely available software. The brains of mature male Wistar rats were used (n = 5). Double immunocytochemical GFAP/GLT-1 staining was performed to obtain fluorescent confocal images. The article proposes the implementation of recent analysis approaches using freely available software to obtain qualitive and quantitative information about the structural features of heterogeneous cells of the central nervous system such as astrocytes. Moreover, the proposed approach provides standardization of data that necessary for comparing the results obtained by different scientific groups.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9c4edbce7f3d7b0f494ca0c1cd0f5488 https://doi.org/10.1134/s1990519x21030081 Zobrazit plný text záznamu Full text from SpringerLink