Chain Dynamics Limit Electron Transfer from Electrode-Bound, Single-Stranded Oligonucleotides
| Autor: | Philippe Dauphin-Ducharme, Netzahualcóyotl Arroyo-Currás, Kevin W. Plaxco, Jacob Somerson, Ramesh Adhikari, Dmitrii E. Makarov, Gabriel Ortega |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Length scale
Oligonucleotide Chemistry Kinetics 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Redox 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials chemistry.chemical_compound Electron transfer General Energy Chain (algebraic topology) Biophysics Physical and Theoretical Chemistry 0210 nano-technology Linker Methylene blue |
| Zdroj: | The Journal of Physical Chemistry C. 122:21441-21448 |
| ISSN: | 1932-7455 1932-7447 |
| DOI: | 10.1021/acs.jpcc.8b06111 |
| Popis: | A wide range of new devices aimed at in vivo molecular detection and point-of-care diagnostics rely on binding-induced changes in electron-transfer kinetics from an electrode-attached, redox-reporter-modified oligonucleotide as their signaling mechanism. In an effort to better characterize the mechanisms underlying these sensors, we have measured the electron-transfer kinetics associated with surface-attached, single-stranded DNAs modified with a methylene blue redox reporter either at the chain’s distal end or at an internal chain position. We find that although the rate of electron transfer from a reporter placed either terminally or internally is independent of chain length for chains shorter than the length scale of methylene blue (and its linker), for longer chains it follows a power-law dependence on length of exponent approximately −2.2. Such behavior is consistent with a diffusion-controlled mechanism in which the diffusion of the DNA-bound reporter to the surface controls the rate of electron tra... |
| Databáze: | OpenAIRE |
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