Ligand-Induced Changes in T Box Antiterminator RNA Stability

Autor:	Jennifer V. Hines, Shu Zhou, Karen D. Jack, Stephen C. Bergmeier, George Acquaah-Harrison
Rok vydání:	2011
Předmět:	Pharmacology Riboswitch RNA Stability Ligand Organic Chemistry RNA Biology Biochemistry Nucleic Acid Denaturation Transcription (biology) Drug Discovery Transfer RNA Biophysics Molecular Medicine Gene
Zdroj:	Chemical Biology & Drug Design. 79:202-208
ISSN:	1747-0277
DOI:	10.1111/j.1747-0285.2011.01274.x
Popis:	The T box antiterminator RNA element is an important component of the T box riboswitch that controls the transcription of vital genes in many Gram-positive bacteria. A series of 1,4-disubstituted 1,2,3-triazoles was screened in a fluorescence-monitored thermal denaturation assay to identify ligands that altered the stability of antiterminator model RNA. Several ligands were identified that significantly increased or decreased the melting temperature (Tm) of the RNA. The results indicate that this series of triazole ligands can alter the stability of antiterminator model RNA in a structure-dependent manner.
Databáze:	OpenAIRE
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