Rapid identification of pseudomonas avellanae field isolates, causing hazelnut decline in central italy, by repetitive PCR genomic fingerprinting
| Autor: | L. Angelucci, U. Marchesi, M. P. Rossi, Maria Teresa Dettori, Marco Scortichini |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Identification methods
Veterinary medicine Physiology Pseudomonas Plant Science Biology Pathogenicity biology.organism_classification Discriminative clustering Microbiology law.invention Rapid identification Pseudomonas avellanae law Genetics Agronomy and Crop Science Pathogen Polymerase chain reaction |
| Zdroj: | Journal of Phytopathology. 148:153-159 |
| ISSN: | 1439-0434 0931-1785 |
| DOI: | 10.1046/j.1439-0434.2000.00481.x |
| Popis: | Pseudomonas avellanae is the main cause of hazelnut (Corylus avellana L.) decline, the so called ‘moria’, in central Italy where it has already killed more than 30 000 trees. Its current identification is very long requiring biochemical, physiological and nutritional tests as well as pathogenicity tests and takes not less than 6 months for its completion. In the present study the reliability of the repetitive polymerase chain reaction (rep-PCR) technique for a rapid and accurate identification of such a pathogen was compared with the traditional identification method. In order to assess the variability of the pathogen, REP, BOX and ERIC primer sets were used in preliminary work to generate genomic fingerprints of 60 P. avellanae reference strains previously isolated from different areas of hazelnut cultivation. ERIC primers yielded the most discriminative clustering of strains that were grouped according to their geographic origin. Sixty field isolates collected from hazelnut orchards of central Italy, planted with different cultivars, during the years 1996–98 were submitted to either the traditional identification methods or to rep-PCR by using ERIC primers. The latter technique accurately identified all the isolates that were also identified by the traditional methods. Whole-cell protein analysis by means of sodium dodecyl sulphate-polyacrylamide gel electrophoresis confirmed this achievement. Rep-PCR can be successfully adopted for the rapid and accurate identification of P. avellanae in central Italy and it constitutes a very useful tool for the sanitation of the area. Zusammenfassung Eine schnelle Bestimmung von Pseudomonas avellanae-Feldisolaten, dem Erreger einer HaselnuIapoplexie in Zentralitalien, durch rep-PCR genomisches Fingerprinting |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|